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Strain Name
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NOD.CB17-Prkdcscid Il2rgtm1Bcgen Igs2tm1(HLA-A2.1-B2M/HLA-A11.1)Bcgen
H2-K1tm1Bcgen H2-D1tm1Bcgen/Bcgen
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Common Name
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B-NDG hB2M/HLA-A11.1 mice plus
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Background
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B-NDG
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Catalog number
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112474
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Aliases
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IMD43; HLAA
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Protein expression analysis
Strain specific B2M expression analysis in wild-type B-NDG mice and homozygous B-NDG hB2M/HLA-A11.1 mice plus by flow cytometry. Splenocytes, blood cells and bone marrow were collected from wild-type B-NDG mice and homozygous B-NDG hB2M/HLA-A11.1 mice plus. Protein expression was analyzed with anti-mouse B2M antibody (Biolegend, 154504) and anti-human B2M antibody (Biolegend, 395712) by flow cytometry. Mouse B2M was detectable in wild-type mice but not in homozygous B-NDG hB2M/HLA-A11.1 mice plus. Human B2M was exclusively detectable in homozygous B-NDG hB2M/HLA-A11.1 mice plus but not in wild-type mice.
Strain specific HLA-A expression analysis in wild-type B-NDG mice and homozygous B-NDG hB2M/HLA-A11.1 mice plus by flow cytometry. Splenocytes, blood cells and bone marrow were collected from wild-type B-NDG mice and homozygous B-NDG hB2M/HLA-A11.1 mice plus. Protein expression was analyzed with anti-mouse H-2Kb/H-2Db antibody (Biolegend, 114613) and anti-human HLA-A,B,C antibody (Biolegend, 311406) by flow cytometry. Mouse H2-K1/H2-D1 was detectable in wild-type mice but not in homozygous B-NDG hB2M/HLA-A11.1 mice plus. Human HLA-A was exclusively detectable in homozygous B-NDG hB2M/HLA-A11.1 mice plus but not in wild-type mice.
Frequency of leukocyte subpopulations
Frequency of leukocyte subpopulations in bone marrow, spleen and blood by flow cytometry. Bone marrow, splenocytes and blood cells were isolated from B-NDG mice and homozygous B-NDG hB2M/HLA-A11.1 mice plus (male,7-week-old, n=3). A. Flow cytometry analysis of the immune cells was performed to assess the frequency of leukocyte subpopulations. Frequencies of T cells, B cells, NK cells, DCs, neutrophils, monocytes and macrophages in B-NDG hB2M/HLA-A11.1 mice plus were similar to those in B-NDG mice, demonstrating that humanization of HLA-A11.1 does not change the frequency or distribution of these cell types in bone marrow, spleen and blood when compared with those in B-NDG mice. Values are expressed as mean ± SEM.
